|Cells||Cor.4U® human iPS-derived cardiomyocytes|
|Species||Human iPS cells of 26y/o Caucasian female|
|Service Type||functional / phenotypic analysis|
|Delivery||A study protocol will be sent to initiate the study. Results are sent as draft and final study report.|
|Timelines||Experiment run time: 1 week per plate
Draft report: within 4 weeks
- Predictive, relevant and robust 96-well assay system based on integrated cell culture model representing primary-like cardiac physiology
- Analysis of both acute and long-term effects on cardiomyocyte beating and overall cell health; combination with other read-outs of cell viability and cytotoxicity possible
- Time- and cost effective assessment of compound safety accelerating lead selection
CardioEffect combines Cor.4U® human cardiomyocytes with impedance technology (xCELLigence RTCA Cardio, ACEA Biosystems) to provide a homogenous and standardized assay system for in vitro detection of compound effects on cell beating. This includes changes of spike amplitude, beating frequency, induction of arrhythmia, and beating arrest.
CardioEffect has been validated in house using 24 drugs with known cardiac safety profiles. Through years of service, Axiogenesis has experience with hundreds of compounds from a variety of classes and indications. Reference compounds are included on each assay plate for quality assurance. In addition to phenotypic assessment of contractile motion, this assay provides a surrogate measure of cytotoxicity via the “Cell Index”.
CardioEffect provides an ideal tool to assess cardiomyocyte function, safety pharmacology / toxicity effects of drug candidates, etc. on human cardiac myocytes in a label-free, real time assay. Understanding cardiotoxic liabilities of compounds in early stages of drug development establishes confidence to move lead compounds towards pre-clinical and clinical development.
The CardioEffect Screening has been validated in house using 24 drugs with known cardiac safety profile. Reference compounds are included on each assay plate to ensure reproducible results.