- Physiological co-culture of CNS neurons and astrocytes
- Stable culture over 5 weeks; neural network formation as of day 18
- Suitable as seizure liability model given long-term synchronous network activity and reactivity to seizure-active compounds
CNS.4UTM represent a highly physiological in vitro co-culture model of neurons and astrocytes for a range of drug development, neurotoxicity and disease modeling applications. CNS.4UTM comprise two key cell types of the human central nervous system (CNS): neurons (glutamatergic, GABAergic and dopaminergic) and astrocytes. The cells are simultaneously generated from neural precursor cells and recapitulate the interplay between excitatory and inhibitory neurons. Their rapid formation of neuronal networks, prolonged viability in culture, and availability in unlimited volumes, makes CNS.4UTM advantageous compared to currently used primary cell and animal models for neuroscience-related applications. CNS.4UTM represent an ideal tool for early & late drug discovery for neurodegenerative disease and epilepsy, in vitro neurotoxicity assays, and disease modeling, through electrophysiological, metabolic and imaging-based assays. Axiogenesis is member of the FDA - HESI Neurotox for seizure liability; CNS.4UTM will be validated through this initiative.
CNS.4U synchronous activity is maintained over 5 weeks in culture
CNS.4U are suitable for seizure liability