Cells Smac.4M® murine ESC derived smooth muscle cells
Species Mouse (strain 129S2/SvPas)
Reporter Gene RFP
Purity 100 %
Package content 1 vial Smac.4M® RFP 1M, culture medium, puromycin, coating solution, disposable Neubauer chamber, cell viability staining
Delivery Cryopreserved
  order
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Smac.4M® Murine smooth muscle cells

Smac.4M® cells are single, spindle-shaped, primary-like smooth muscle cells derived from mouse ES cells. They are a homogeneous source of cells that are 95 % pure and express smooth muscle cell-specific markers like calponin, transgelin, desmin, acta2, and myh11. Presence of all relevant ion channels has been demonstrated in Smac.4M® cells. Contraction of Smac.4M® cells can be induced by angiotensin II, endothelin I, and potassium chloride. Smac.4M® cells express a red fluorescent reporter protein and are available in different formats. They are useful to pharmaceutical industry and to academia for drug profiling and target discovery, as well as for prediction of organ-specific toxicity. Smac.4M® cells display very little batch-to-batch variation and are easily integrated into high-content screening assays.

Advantages Smac.4M® smooth muscle cells
  • High purity – 95 % smooth muscle cells, no de-differentiation.
  • Suitable for contraction analysis (inducible).
  • Responsive to L-type Ca2+ channel mediators.
  • Comparable behavior to aortic smooth muscle cells.
  • Well characterized, authentic und reproducible cellular environment.
  • Consistent performance – large lot sizes with minimal lot-to-lot variation.
  • Predictive model – relevant smooth muscle physiology.
  • Stable and convenient format – cryopreserved in vials or 96-well plates.
  • Standard protocols for many applications available.