Recordings of extracellular potentials from microelectrode arrays (MEA) offers non-invasive and long-term monitoring of monolayers of spontaneously contracting cardiomyocytes. Simultaneous current clamp and MEA recording from the same cardiomyocyte culture demonstrate comparable components of the waveforms in both assay systems, as well as similar modulation of the analyzed parameters by cardioactive drugs.
Spontaneously-beating Cor.4U® human cardiomyocytes have electrophysiological properties typical for primary cardiac myocytes, e.g. voltage-gated ion currents, or ß-adrenergic and muscarinic receptors.
The combination of the MEA technology with Cor.4U® human iPS cell-derived cardiomyocytes provides an ideal non-invasive and label-free tool to assess the pharmacology and safety of drug candidates.
- Non-invasive, long-term monitoring of cardiomyocytes.
- Primary-like human cardiomyocyte phenotype in a highly standardized, convenient cell model .
- Assessment of both direct and indirect effects on cardiac electrophysiology
To perform experiments with Cor.4U@ human cardiomyocytes using the MEA technology has been optimised for the Axion Maestro (http://www.axionbiosystems.com/products/mea-systems) and the Multi Channel Systems MEA instruments (http://www.multichannelsystems.com).