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Calcium flux analysis/Fluo-4 FLIPR assay

High throughput assay systems are the basis for drug discovery and target validation in the pharmaceutical industry. Systems like the fluorometric plate reader (FLIPR® or FLIPRtetra®) from Molecular Devices provide the essential assay environments to conduct large scale and automated screening campaigns.

Cardiac ion channels are typical targets to assess the efficacy or side effects of potential drugs. The ‚gold standard’ to analyze effects on ion channels is the patch clamp technique. Although the throughput of automated patch clamp devices is steadily increasing it is still impossible to screening large compound libraries with these systems.

The FLIPR® Fluo-4 Assay provide a rapid and reliable fluorescence-based method to detect changes in intracellular calcium ion concentrations and Cor.At® cardiomyocytes have been successfully cultured with stable and rhythmic beating for up to 1 week in high density microtiter plates and applied in to the Fluo-4 assay.

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Advantages: Fluo-4 FLIPR assay with Stem Cell-derived Cardiomyocytes
  • HTS assay with primary-like and pure cardiomyocytes.
  • Monitoring of intracellular Ca2+ transients.
  • Predictive for a variety of cardiac targets (cardiac GPCRs, ion channels, transporters, etc.).
  • Ideal assay for lead optimization.